| Detection of Shrimp Virus |
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EBPL deals with IQ 2000 viral detection systems, the best PCR diagnostic tests kits available for detection of shrimp viruses produced by Farming Intelligene Tech Corporation, Taiwan . EBPL incorporation with FITC provides diagnostic kits for all viruses commonly found in black tiger shrimp ( Penaeus monodon ), White shrimp ( Penaeus indicus ), American white shrimp ( Penaeus Vanamai ) and fresh water prawn ( Machrobrachium rosenbergi ) including the recent developed IMNV.
IQ2000T WSSV Detection and Prevention System is the best molecular diagnostic system for direct WSSV diagnosis. For the development of an effective diagnosis system, a patented segment of WSSV* was selected as the target for WSSV diagnosis. This system can differentiate infected samples into 4 different levels of infection: very light, light, medium, and severe infection. To ensure accurate and reliable results, internal control and quantified positive standard are intrinsic to the system. In addition, this system was proven to be very effective for detection of different WSSV strains reported from different areas around the world. Since 1998, this system has being accepted by many large-scale integrated shrimp farms to prevent white spot disease with great success. * US patent Nos. 9,099,062, 8,800,038, patent pending for Thailand , China , EU and Taiwan . A Licensing Agreement was signed with National Science Council in 1998, which allows Farming IntelliGene to commercialize the WSSV research done by Dr. Guang-Hsiung Kou and Dr. Chu-Fang Lo for WSSV diagnosis. Specifications:
Results: Typical diagnostic results generated by IQ2000T WSSV Detection and Prevention System.
Lane 1: sample with severe WSSV infection
TSV was first reported in Ecuador . 1992, and caused severe impact on the L. vannamei culture industry till now. This single strained RNA virus spread widely not only in America but also in Asia where L.vannamei is becoming popular. Recently, TSV infection was also reported on P. monodon , L.stylirostris and P. japonicus . Therefore, TSV screening and prevention are very important for shrimp farming industry worldwide. The IQ2000 T TSV Detection and Prevention System is the best molecular diagnosis system for Taura Syndrome Virus. A very thorough analysis was conducted to ensure TSV strains isolated from different geographic regions could be detected by this system. As RNA is more difficult than DNA in extraction and handling, we designed a RNA internal control for this system to avoid the potential false negative result. To date, IQ2000 T TSV Detection and Prevention System is the only TSV detection system available on the market with internal control and semi-quantitative design. Specifications:
Results: Typical diagnostic results generated by IQ2000 TSV Detection and Prevention System.
Lane 1: TSV plasmid P(+) control, 2000 copies/reaction
The IQ2000T YHV/GAV Detection and Typing System for penaeid shrimps is a commercial molecular diagnostic system for direct detection of these two similar viruses. It can detect and differentiate the prototype of these two viruses and provide semi-quantitative results. The knowledge about the nature of YHV, GAV and their RNA sequences were originated from the research of CSIRO, Australia , and BIOTEC, Thailand . A licensing agreement was signed in 2001 between Farming IntelliGene and CSIRO/BIOTEC to commercialize this technology for detection of YHV/GAV. * According to the recent research results, other YHV/GAV related viruses have been found in some Asian countries. Due to the lack of sequence information, IQ2000 YHV/GAV Detection and Typing System may not be able to detect these YHV/GAV related viruses, or may cross-react with these viruses. The impact and severity of these newly discovered viruses on shrimp farming and their relationship with YHV/GAV is still under investigation. Specifications:
Result: Typical diagnostic results generated by IQ2000 YHV/GAV Detection and Typing System.
Lane 1: YHV positive standard, 2000 copies/reaction
IHHNV is a notorious pathogen, which can cause mass mortality for L.stylirostris , and RDS (runt and deformity syndrome) for L.vannamei . Recent research also points out that IHHNV is related to muscle whitening syndrome, and it may affect the survival rate of domesticated P.monodon . IQ2000 IHHNV Detection and Prevention is designed for direct diagnosis of Infectious Hypodermal and Hematopoietic Necrosis Virus. While designing this system, the most conserved region of IHHNV was discovered, and was used as the target for IHHNV detection. Due to the IHHNV infection is always presented in much higher concentration than other viruses, a single step PCR amplification is sufficient for its detection. To ensure accurate and reliable results, this system also contains internal control and quantified positive standard. Semi-quantitative designs differentiate 3 different levels of infection; this provides useful information for shrimp farm health management. Specifications:
Results: Typical diagnostic results generated by IQ2000 IHHNV Detection and Prevention System.
Lane 1: sample with severe IHHNV infection Applications:
What is IQ2000 MultiVir System? IQ2000 MultiVir System is a "3 in 1" multiviral detection system.
Who should use IQ2000 MultiVir System?
What are the advantages of IQ2000 MultiVir System?
Interpretation of IQ2000 WIT MultiVir System
Experimental Procedure
IQ2000 T HPV Detection and prevention System is a molecular diagnostic system designed for direct diagnosis of HPV. The system can differentiate infected samples into 3 levels: light, medium and severe infection. Internal control is intrinsic to this system to ensure correct diagnosis. The IQ2000 T HPV Detection and Prevention System can detect different HPV strains from different areas, such as strains isolated from Thailand , Australia , Korea , Mexico , Panama and Taiwan etc. Specifications:
Results: Typical diagnostic results generated by IQ2000 T HPV Detection and Prevention System.
Lane 1: standard 1, 20000 copies/reaction Applications:
Penaeus monodon -type baculovirus (MBV) is one of the earliest found shrimp viruses, but its impact on shrimp farming has yet to be further studied. MBV was considered to be one of the most critical fatal shrimp diseases for P. monodon in the past, but recent researches have shown no direct association of such disease to the cause of larger failure of shrimp culture industry in respect of growth retardation, deformation, and mortality rate. Nevertheless, MBV is still a considerable issue for hatchery management as it may affect the survival rate of PLs. The consequences of severe MBV infection not simply affect the PLs survival rate, but the performance of PLs also turns out to be worse than those lightly infected or negative ones. As the major transmission path of MBV is horizontal, the status of PLs MBV infection has been taken as a major indicator of hatchery hygiene in some areas. The comparison of MBV infected samples from several Asian countries has indicated that the variation of nucleic acid sequence is higher than expected. For example, within the published sequence by Craig R. Belcher (J. Virol. Method 74, 1998, p21-29), the homology of the sample from Taiwan is only 91.8%; 92.3% for sample from Thailand , 92.1% for sample from Indonesia , and 94.1% for sample from India . These sequence variations may cause false negative results by following Craig's protocol. Farming IntelliGene has been researching on MBV continuously. This newly released version of IQ2000 T MBV Detection and Prevention System has the broadest coverage of strains in comparison with other known products in the market and has also inherited the built-in internal control design and semi-quantitative data format from a chain of our IQ2000 T series products. Moreover, we always ensure that our detection system is updated as soon as we have any advanced research results in order to provide the best detection tool for the users of IQ2000 T series products . Specifications:
Results: Typical diagnostic results generated by IQ2000T MBV Detection and Prevention System.
Lane 1: standard 1, 20000 copies/reaction Applications:
Infectious Myonecrosis Virus (IMNV) attacks crops of L. vannamei , and its first appearance in Brazil , 2002. And it is not until February, 2004, that Dr. Donald V. Lightner, from the University of Arizona , US, officially confirmed the discovery of the new virus. The late discovery and the lack of information have caused severe damages to the Brazilian farming industry. In cooperation with the research team leaded by Dr. Donald V. Lightner, Farming IntelliGene Tech Corp. has created a highly accurate detection system for IMNV, a new product in IQ2000T viral detection series. It has inherited all the same features from IQ2000T series, such as internal control and semi-quantitative design. The first design eliminates false negative possibilities, and the second one measures the degree of infection. Specifications:
Results: Typical diagnostic results generated by IQ2000 IMNV Detection and Prevention System. Lane 1: IMNV plasmid DNA standard, 2000 copies/reaction Applications:
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