Detection of Sea Food Pathogen using PCR


The recent advances in molecular biology and shrimp biotechnology enable in detecting most of the pathogens causing serious threat to food safety. The traditional ways of microbiological analysis limiting to diagnosis of all the dangerous pathogens at time due to the critical steps of culture and isolation while handling more then one species. It is also a time consuming process due to the nature of some slow growing species such as salmonella. PCR (Polymerised Chain Reaction) a vital tool in biotechnology normally used for detection of virus in animals and plants.

Now PCR technology is also being used for detecting most of the pathogens causing serious hazards in processing industry. Dr.Food TM Kit is designed keeping in view of the most important nine targeted pathogens of marine food processing industry. The sensitive nature of PCR and the consistence performance of Dr.Food TM Kit enable to detect the targeted pathogens accurately in a short period of time as quick as 24 hours.

Advantages of PCR detection of Pathogens using Dr.Food™ Kit

  • Nine targeted bacteria can be detected in each sample by one single PCR test.
  • At Time a (24-96) number of samples can be taken for detection of targeted bacteria.
  • Quick and accuracies in getting result save time and money.
  • Sensitivity and consistencies in performance of Dr.Food TM Kit can improve food safety under HACCP plane.
  • The process of PCR indulges automatic result interpretation.
  • Dr.Food TM Kit has an internal control for conformation of each targeted species.
  • No electrophoresis required and easy to practice.
  • The critical steps of culture & isolation in handling more then one species can be easily shorted out.

Sensitivity of Dr.Food Kit and Targeted Pathogens

Pathogen

Sensitivity cfu/mL

Pathogen

Sensitivity cfu/mL

Staphylococcus aureus

10 9 ~104

Listeria onocytogenes

10 9 ~10 4

Escherichia coli

108 ~10 4

Salmonella spp.

10 9 ~10 4

Yersinia enterocolitica

10 8 ~10 4

Shigella spp.

10 8 ~104

Bacillus cereus

108 ~10 3

Vibrio spp.

108 ~104

Clostridium perfringens

108 ~103

 

 


Process involved in using Dr.Food Kit

Sl. No

Process

1

Sample Preparation

2

Enrichment

3

DNA Extraction

4

PCR

5

Biochip Hybridization

6

Coloration

7

Result interpretation


Presentation of Dr.Food
Kit


Pathogen Detection By 3 Well Chip
Result Interpretation of 3 Well Chip
   
Pathogen Detection By 96 Well Chip
Equipment Required

 
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