The most common viruses found in marine fishes of recent time are:

• Viral Nervous Necrosis Virus (VNN)
• Koi Herpesvirus (KHV)
• Iridovirus

Viral nervous necrosis disease (VNN) has been reported in marine fishes such as parrot fish, groupers, flounders, sea bass, turbot, and striped jack since 1990. This disease caused high mortalities in the affected animals that were characterized by vacuolation in the retinal and brain tissue. Now, the causative agents of VNN were identified as a member of the family Nodaviridae. Even the nucleic acid sequences between fish nodaviruses are distinguishable, but they are closely related to each other in the coat protein region. IQ2000 T VNN Detection and Prevention System is designed by this conserved region and it can detect the nervous necrosis viruses on striped jack (SJNNV), redspotted grouper (RGNNV), barfin flounder (BFNNV), Japanese flounder (JFNNV), and tiger puffer (TPNNV), and most kinds of the groupers in Taiwan.

Specifications:

• Packing size: 200 reactions.
• Detection Limit: 10 copies/reaction.
• Quantitative capability: 3 different levels of infection can be differentiated.
• Sample throughput: for 40 samples, from sample preparation to final results require 4.5 to 5 hours.
• Built-in internal control system: eliminate false negative results from failed extraction or reaction.
• Quantified positive standard: monitoring the sensitivity of reaction.

Results:

Typical diagnostic results generated by IQ2000 T VNN Detection and Prevention System.

Lane 1: standard 1, 2000 copies/reaction
Lane 2: standard 2, 200 copies/reaction
Lane 3: standard 3, 20 copies/reaction
Lane 4: ddH2O
Lane 5: sample with severe VNN infection
Lane 6: sample with light VNN infection
Lane 7: VNN negative sample
Lane M: molecular weight marker, 848 bps, 630 bps, 333 bps

Applications:

• Suitable for fresh, frozen, and ethanol preserved fish brain, blood, and eye samples.
• Ideal for SPF animal selection, Evaluation of culture environment, Grow-out monitoring, and Quarantine service.

Koi carp has long been regarded as an important tropical ornamental fish of very high added value in the fish farming industry in Asia .  In recent years, the industry has been suffering from a severe blow through the infection of Koi Herpesvirus-KHV.  The outbreak of KHV is found in some Asia countries, such as Taiwan , Hong Kong and Indonesia , as well as in Israel and Europe .   KHV disease process is highly contagious and is most characteristic by causing massive morbidity and mortality rate ranging between 80%-90%.  According to an Indonesian research analysis (Agus Sunarto et. al , paper presented in 5 th Symposium on Disease in Asian Aquaculture, 14-28 Nov. 2002, Gold Coast, Australia), the process of KHV infection in East Java has affected over 5000 fish farmers and caused a loss of US$0.5 million from March to October in 2002.   As the trading business of ornamental fish among Asian countries has been very popular, the prevention of KHV exposure and quarantine is no doubt a key issue in the protection of local koi farming industry.

IQ2000 T KHV Detection and Prevention System is the first viral diagnostic system specially designed for ornamental fish by Farming IntelliGene.  This system has adopted the design of Nested PCR and also inherited the reliability, sensitivity and experience from a range of IQ2000 T viral diagnostic kits for shrimp and fish viral infections.  IQ 2000 KHV has effective and reproducible detection limit of 10 viral copies while in most cases digit copy of virus can also be detected.  IQ2000 T KHV offers you the best tool for the prevention of KHV infection.

Specifications:

• Packing size: 200 reactions.
• Detection Limit: 10 copies/reaction.
• Quantitative capability: 3 different levels of infection can be differentiated.
• Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.
• Built-in internal control system: eliminate false negative results from failed extraction or reaction.
• Quantified positive standard: monitor the sensitivity of detection.

Results:

Typical diagnostic results generated by IQ2000 T KHV Detection and Prevention System.

Lane 1: standard 1, 2000 copies/reaction
Lane 2: standard 2, 200 copies/reaction
Lane 3: standard 3, 20 copies/reaction
Lane 4: ddH2O
Lane 5: sample with severe KHV infection
Lane 6: sample with light KHV infection
Lane 7: KHV negative sample
Lane M: molecular weight marker, 848 bps, 630 bps, 333 bps

Applications:

• Suitable for fresh, frozen, and ethanol preserved fish tissue samples.
• Ideal for SPF animal selection, Evaluation of culture environment, and Quarantine service.

Iridoviruses have caused ecological and economic impacts on marine fish farming in recent years. Pathogenic Iridovirus was isolated from diseased grouper ( Epinephelus hybrid ), giant seaperch ( Lates calcarifer ), largemouth bass ( Micropterum salmoides ), king grouper ( Epinephelus lanceolatus ), spotted butter fish ( Scatophagus argus ), yellow-wax pomfrat ( Trachinotus blochii ), goldlined seabream ( Sparus sarba ), humpback grouper ( Chromileptes altivelis ) and Mangrove red snapper ( Lutjanus argentimaculatus ). The virus cause s severe mortality in cultured grouper fry.

IQ2000T Irido Detection and Prevention System is the first viral diagnostic system specially designed for marine fish by Farming IntelliGene.  This system has adopted the design of Nested PCR and also inherited the reliability, sensitivity and experience from a range of IQ2000T viral diagnostic kits for shrimp and fish viral infections.  IQ2000T Irido has an effective and reproducible detection limit of 10 viral copies while in most cases digit copy of virus can also be detected.  IQ2000T Irido offers you the best tool for the prevention of Iridovirus infection.

Specifications:

• Packing size: 200 reactions.
• Detection Limit: 10 copies/reaction.
• Quantitative capability: 3 different levels of infection can be differentiated.
• Sample throughput: for 40 samples, from sample preparation to final results require 3 .5 to 4 hours.
• Built-in internal control system: eliminate false negative results from failed extraction or reaction.
• Quantified positive standard: monitoring the sensitivity of reaction.

Results:

Typical diagnostic results generated by IQ2000T Irido Detection and Prevention System.

Lane 1: standard 1, 2000 copies/reaction
Lane 2: standard 2, 200 copies/reaction
Lane 3: standard 3, 20 copies/reaction
Lane 4: sample with severe Iridovirus infection
Lane 5: sample with light Iridovirus infection
Lane 6: Iridovirus negative sample
Lane 7: ddH 2 O
Lane M: molecular weight marker, 848 bp, 630 bp, 333 bp

Applications:

• Suitable for fresh, frozen, and ethanol preserved fish brain, blood, and eye samples.
• Ideal for SPF animal selection, e valuation of culture environment, g row-out monitoring, and quarantine service.